Tunicamycin (home):

1: Neurochem Res 2002 Dec;27(12):1669-75  

Effect of 2-fluoropalmitate, cerulenin and tunicamycin on the palmitoylation and intracellular translocation of myelin proteolipid protein.

DeJesus G, Bizzozero OA.

Department of Cell Biology and Physiology, University of New Mexico-School of Medicine, Albuquerque, New Mexico 87131-5218, USA.

We have investigated the effect of documented protein palmitoylation inhibitors on the fatty acylation and intracellular transport of myelin proteolipid protein (PLP). To this end, brain slices from 20-day-old rats were incubated with either [3H]palmitate or [3H]leucine in the presence or absence of various concentrations of 2-fluoropalmitate (FP), cerulenin (CER), or tunicamycin (TM). FP (> or = 10 microM) decreased the cellular uptake of [3H]palmitate and consequently reduced the labeling of palmitoyl-CoA, glycerolipids and PLP. CER (> or = 1 mM) reduced the palmitoylation of PLP with a concomitant decline in protein thiols. Consistent with being a fatty acyl-CoA analogue, TM (> or = 200 microM) diminished the palmitoylation of PLP and lipids while increasing the amount of [3H]palmitoyl-CoA. Although both CER and TM decreased protein palmitoylation, only the latter affected the appearance of newly synthesized PLP into myelin. Because TM, but not CER, also reduced the formation of lipids, it is concluded that palmitoylation is not required for intracellular transport. Finally, comparison of the effect of TM in brain slices and in a cell-free system suggests that palmitoylation of PLP in whole cells may be an enzymatic process.

PMID: 12515321






www.linkstoyou.com This page was done by a student of Bioinformatics, Open University Jerusalem Israel, in a homage to Fermentek and to Dr Berend.
 4mg  noneto biocompare formulae omd ToxicPlants DictCellBio MolBioDict ( test  position )
BioChemical Suppliers
A23187 Actinomycin Aflatoxin Anisomycin Aphidicolin Ascomycin


Cerulenin Chromomycin Geldanamycin Cyclopiazonic acid
Cytochalasin Forskolin Fumagillin Fumonisin Hypericin K252a
KT5823 Mitomycin Nigericin Ochratoxin Oligomycin Mycophenolic acid
Paclitaxel Patulin Paxilline Penitrem Puromycin Penicillic acid
Radicicol Rapamycin Staurosporine Sterigmatocystin Thapsigargin Tunicamycin
 Verruculogen  Wortmannin